Moore , Stanford

Moore , Stanford
(1913–1982) American biochemist
Moore, who was born in Chicago, Illinois, graduated in chemistry from Vanderbilt University in 1935 and received his PhD from the University of Wisconsin in 1938. He then joined the staff of the Rockefeller Institute, spending his entire career there and serving as professor of biochemistry from 1952 onward.
One of the major achievements of modern science has been the determination by Frederick Sanger in 1955 of the complete amino acid sequence of a protein. Sanger's success with the insulin molecule inspired Moore and his Rockefeller colleague, William Stein (1911–1980), to tackle the larger molecule of the enzyme ribonuclease. Although their work was lightened by the availability of techniques pioneered by Sanger the labor involved was still immense until eased by their development of the first automatic amino-acid analyzer.
They inserted a small amount of the amino-acid mixture into the top of a five-foot column containing resin. They then washed down the mixture using solutions of varying acidity. The individual amino acids travel down the column at different rates depending on their relative affinity for the solution and for the resin. It is possible to adjust the rates of travel so that the separate amino acids emerge from the bottom of the column at predetermined and well-spaced intervals. The colorless amino acids were then detected with ninhydrin, a reagent that forms a blue color on heating with proteins and amino acids. A continuous plot of the intensity of the blue color gives a series of peaks, each corresponding to a certain amino acid with the area under the peak indicating the amount of each.
By the end of the 1950s Moore and Stein had not only established the sequence of ribonuclease but they were also able to indicate the most likely active site on the single-chained molecule. For this work they shared the 1972 Nobel Prize for chemistry with Christian Anfinsen.

Scientists. . 2011.

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  • Moore, Stanford — Moore , Stanford …   Scientists

  • Moore, Stanford — born Sept. 4, 1913, Chicago, Ill., U.S. died Aug. 23, 1982, New York, N.Y. U.S. biochemist. He shared a 1972 Nobel Prize with Christian Anfinsen (1916–95) and William Stein (1911–80) for research on the molecular structures of proteins. He is… …   Universalium

  • Moore, Stanford — ► (1913 82) Bioquímico estadounidense. Fue premio Nobel de Química en 1972, compartido con Ch. B. Anfinsen y W. H. Stein, por sus estudios sobre la biosíntesis de la nucleasa. * * * (4 sep. 1913, Chicago, Ill., EE.UU.–23 ago. 1982, Nueva York,… …   Enciclopedia Universal

  • Moore — Stanford …   Scientists

  • Stanford Moore — (* 4. September 1913 in Chicago; † 23. August 1982 in New York City) war ein US amerikanischer Biochemiker. Er erhielt 1972 den Nobelpreis für Chemie für seine Arbeiten zur chemischen Struktur und zur katalytischen Wirkung des aktiven Zentrums… …   Deutsch Wikipedia

  • Moore — Moore, George Moore, Henry Moore, Stanford Moore, Thomas * * * (as used in expressions) Grimké, Sarah (Moore) y Grimké, Angelina (Emily) Kennedy, Edward M(oore) David John Moore Cornwell Moore, Archie Moore, Brian Moore, Clement Clarke …   Enciclopedia Universal

  • Moore — /moor, mawr, mohr/, n. 1. Archibald Lee (Archie), born 1916?, U.S. boxer. 2. Brian, born 1921, U.S. novelist. 3. Clement Clarke, 1779 1863, U.S. scholar and writer. 4. Douglas Stuart, 1893 1969, U.S. composer. 5. Eliakim Hastings /i luy euh kim/ …   Universalium

  • Stanford — /stan feuhrd/, n. 1. (Amasa) Leland, 1824 93, U.S. railroad developer, politician, and philanthropist: governor of California 1861 63; senator 1885 93. 2. a male given name. * * * (as used in expressions) Moore Stanford Stanford Amasa Leland… …   Universalium

  • Stanford — (as used in expressions) Moore, Stanford Stanford, (Amasa) Leland White, Stanford …   Enciclopedia Universal

  • Stanford Moore — (4 septembre 1913 à Chicago 23 août 1982 à New York) était un biochimiste américain. Il est colauréat avec William Howard Stein d une moitié du prix Nobel de chimie de 1972[1]. Sommaire 1 Biographie …   Wikipédia en Français

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